
Porcine Erythropoietin ELISA Kit with High Spesificity and Sensitivity
Cat.No E0429Po
Reagent Provided
Components | Quantity |
Standard Solution (2400ng/L) | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (30x) | 20ml x1 |
Biotinylated Porcine EPO Antibody | 1ml x1 |
User Instruction | 1 |
Plate Sealer | 2 pics |
Zipper bag | 1 pic |
Specimen Collection
Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.
Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.
Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.
Shanghai Korain Biotech Co., Ltd
Shanghai Korain Biotech Co. was built in 2010. As an creator in reagents and tools for life science, Korainbio provide researchers with tools and scientific support including 30,000 antibodies, 1000+ proteins and 5000 ELISA kits. We aim to be a leading provider with world-class level for the researchers all over the world. Our products line covers a set of research areas including Immunology, Neuroscience, Cancer, Kinases, Phosphatases and Cell Biology.
Bioassay Technology Laboratory (BT Lab) as a leading brand of Korainbio focused on helping researchers to optimize life science work and serves as a provider of test and developing services, including elisa tests, WB test and antigen development. Founding in 2010, our strategic focus has been on the development of enabling technologies in the research, development, manufacture and marketing of innovative immuno products and services based on molecular technologies.
Korain’s reagents are supported by superior processional team and a quality management system that is certified for CE and ISO. Our product development program for use in a variety of applications including:
ELISA and immunohistochemistry
About BT Lab
As a leading brand of Korainbio, Bioassay Technology Laboratory (BT Lab) offers a variety of cost-effective ELISA kits and sets to measure cytokines, chemokines, and soluble biomarkers consistently and reliably. Sandwich kit and Competitive that provide the core reagents our selection of ELISA products meets the demand of ELISA beginners to experts alike at a very economical price.
Worldwide Distributors
ELISA Kit Categories
Sandwich Kit
Sandwich kits are fully validated and ready-to-use, containing 96-
well strip plates pre-coated with capture antibody to detect sample
antigen.
Competitive Kit
In a competitive ELISA assay, sample antigen and labeled antigen
compete for capture antibody binding. The more target protein there
is in the sample, the less labeled antigen will be captured and the
weaker the signal.
Qualitative Kit
Qualitative results provide a simple positive or negative result
for a sample. In quantitative ELISA, the optical density (OD) of
the sample is compared to a standard curve, which is typically a
serial dilution of a known-concentration solution of the target
molecule. It is suitable for detecting small antigens.
Advantages of our Sandwich kit
Without Diluting Sample
Streptavidin-biotin system
Washing Step
Pre-Coated plate
What We Provide
What We Aim to
If you’re interested in working with us to expand the market, please feel free to contact us by: save@bt-laboratory.com